---
title: A Label-free Method to Determine the Glycolytic State of T cells for CAR T Generation
description: Discover a label-free method using Ghost Cytometry to sort CAR T cells with low glycolytic activity, ensuring cellular viability for downstream applications.
---

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**A Label-free Method to Determine the Glycolytic State of T cells for CAR T Generation**

![A Label-free Method to Determine the Glycolytic State of T cells for CAR T Generation](https://info.thinkcyte.com/hubfs/Assets/Screenshot%202025-01-17%20101221.jpg "A Label-free Method to Determine the Glycolytic State of T cells for CAR T Generation")

Introduction:

Studies show that CAR-T cells with lower glycolytic activity yield better anti-tumor outcomes, but current methods to isolate T cell subsets by complex intracellular phenotypes like glycolysis have limitations. Ghost Cytometry offers a morphology-driven method to isolate T cells of different glycolysis states, giving researchers a powerful new tool for cell therapy research.

Summary:

1. Low glycolytic CAR-T cells could be identified by Ghost Cytometry (GC)
2. Low glycolytic CAR T cells identified by GC correlated with microscopy morphology data and standard flow cytometry techniques
3. Ghost Cytometry is an effective technique to isolate CAR T cells with different metabolic profiles for downstream use

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