Introduction:

Macrophages can polarize into M1 and M2 phenotypes, mediating pro- and anti-inflammatory responses, respectively. Isolating populations of polarized macrophages is crucial for understanding tumor progression. VisionSort can isolate pure populations of M1 and M2 macrophages label-free, yielding untouched cells for downstream R&D applications.

Summary:

1. Macrophage subtypes (M1 and M2a) were classified with high accuracy using machine learning
2. Confirmation by both supervised and unsupervised (UAMP) machine learning approaches
3. VisionSort can identify and differentiate between M1 and M2a macrophages, allowing for label-free isolation of phenotypically defined cell from complex mixtures for downstream R&D.

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