---
title: Label-free Macrophage Subtyping
description: Isolate pure M1 and M2 macrophage populations label-free with VisionSort for enhanced understanding of tumor progression and inflammatory responses in research applications.
---

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**Macrophage Subtyping by Morphology**

![Screenshot 2025-01-17 112445](https://info.thinkcyte.com/hubfs/Assets/Screenshot%202025-01-17%20112445.png "Screenshot 2025-01-17 112445")

Introduction:

Macrophages can polarize into M1 and M2 phenotypes, mediating pro- and anti-inflammatory responses, respectively. Isolating populations of polarized macrophages is crucial for understanding tumor progression. VisionSort can isolate pure populations of M1 and M2 macrophages label-free, yielding untouched cells for downstream R&D applications.

Summary:

1. Macrophage subtypes (M1 and M2a) were classified with high accuracy using machine learning
2. Confirmation by both supervised and unsupervised (UAMP) machine learning approaches
3. VisionSort can identify and differentiate between M1 and M2a macrophages, allowing for label-free isolation of phenotypically defined cell from complex mixtures for downstream R&D.

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